Analytical Cytology: Methods for Studying Cellular Form and Function

By Robert C. Mellors | Go to book overview
Save to active project
ketones and for PNA, plasmalogen, phospholipid, esterases, and dehydrogenases. The validity of localization has received close scrutiny for the nucleal stain for DNA-protein and the stains for phosphatases, cholinesterases, and, to a lesser extent, esterases, ϟ-glucuronidase, and oxidative enzymes. From these critical studies have emerged principles and procedures by which staining methods, past and future, can better be evaluated and improved. It is always a temptation, particularly in the enzyme field with its almost limitless numbers of individual enzymes, to attempt the development of new methods. It seems to us, however, that the time has arrived when a new staining method should be subjected to all the tests of reliability used with profit for other methods, before it is proposed for histochemical use. A recent publication by Holt (347) may serve as a model of the critical thought and painstaking work required. Where possible, parallel biochemical and staining studies (251, 241, 163) would appear highly desirable.Important developments may be anticipated in all the areas which we have discussed. We look forward especially to future work on (a) the application of microspectrophotometry to a wider variety of staining methods, (b) the evaluation of cold polyethylene glycols as fixing- dehydrating agents and as embedding media, (c) the use of agents like lead tetraacetate to characterize different types of polysaccharides, (d) the possibility of visualizing ketonic substances, (e) the methods for protein staining presented in preliminary form by Danielli (69), (g) the validity of enzyme localization indicated by the indoxyl and tetrazolium methods, (h) the effectiveness of salts in reducing enzyme diffusion in fresh-frozen sections, (i) the effectiveness of the measures suggested by different investigators to eliminate diffusion of products of enzyme reactions, (j) the usefulness of differences in staining reactions for the classification of esterases (140); and (k) the usefulness of tetrazolium salts and indoxyl salts for visualizing enzyme sites in living cells (114, 152).
References
1. Abolins L., and Abolins A., "Different kinds of acid phosphatase in various cytological structures of the anterior pituitary of the guinea pig", Nature, 164: 455-456 ( 1949).
2. Adamstone F. B., and Taylor A. B. , "The rapid preparation of frozen tissue sections", Stain Technol., 23: 109-116 ( 1948).
3. Albert S., and LeBlond C. P., "The distribution of the Feulgen and 2,4-dinitrophenyl-hydrazine reactions in normal, castrated, adrenalectomized and hormonally treated rats", Endocrinology, 39: 386-400 ( 1946).
4. Allfrey V., Stern H., Mirsky A. E. , and Saetren H., "The isolation of cell nuclei in non-aqueous media", J. Gen. Physiol., 35: 529- 554( 1952).
5. Altmann R., "Die Elementaror"

-2/48-

Notes for this page

Add a new note
If you are trying to select text to create highlights or citations, remember that you must now click or tap on the first word, and then click or tap on the last word.
Loading One moment ...
Project items
Notes
Cite this page

Cited page

Style
Citations are available only to our active members.
Sign up now to cite pages or passages in MLA, APA and Chicago citation styles.

Cited page

Bookmark this page
Analytical Cytology: Methods for Studying Cellular Form and Function
Settings

Settings

Typeface
Text size Smaller Larger
Search within

Search within this book

Look up

Look up a word

  • Dictionary
  • Thesaurus
Please submit a word or phrase above.
Print this page

Print this page

Why can't I print more than one page at a time?

While we understand printed pages are helpful to our users, this limitation is necessary to help protect our publishers' copyrighted material and prevent its unlawful distribution. We are sorry for any inconvenience.
Full screen
/ 32

matching results for page

Cited passage

Style
Citations are available only to our active members.
Sign up now to cite pages or passages in MLA, APA and Chicago citation styles.

Cited passage

Welcome to the new Questia Reader

The Questia Reader has been updated to provide you with an even better online reading experience.  It is now 100% Responsive, which means you can read our books and articles on any sized device you wish.  All of your favorite tools like notes, highlights, and citations are still here, but the way you select text has been updated to be easier to use, especially on touchscreen devices.  Here's how:

1. Click or tap the first word you want to select.
2. Click or tap the last word you want to select.

OK, got it!

Thanks for trying Questia!

Please continue trying out our research tools, but please note, full functionality is available only to our active members.

Your work will be lost once you leave this Web page.

For full access in an ad-free environment, sign up now for a FREE, 1-day trial.

Already a member? Log in now.

Are you sure you want to delete this highlight?