But, while keeping them separate, we can make these fermentations follow each other, although, according to the ideas of Liebig, they are interblended. Take this liquid in which the lactic ferment has grown, and in which there are only lactate of lime and mineral salts in solution: after having heated it to sterilize it, sow there a drop of a liquid in which there has occurred spontaneously a butyric fermentation, and, therefore, one which is almost surely impure. Phenomena anal- ogous to those of alcoholic fermentation occur: a gas is liberated which is no longer a pure carbonic acid, but a mixture of this gas and hydrogen. This mixture has very little odor, because of the absence of sulphuretted hydrogen. These are the indications of a fermentation. Let us see now what is present in the liquid which has become clouded. We find there only motile rods, very agile, with undulating movements, sometimes ranged in a series, like a string of boats, and then motile on their articulations ( Fig. 8, sec. 3 ), which testifies to the fact that they reproduce and multiply by elongating and segmenting across their longer axis; this is the mode of reproduction called fission. When he observed for the first time those organisms which he called vibrios, Pasteur had a great surprise, the trace of which is visible in his note on this subject. The yeast of beer and the lactic ferment were non-motile globules; the butyric ferment was motile, and partook of the nature [animal] of those organisms which Ehren- burg and Dujardin had found in infusions. O the power of words! Nothing was more natural than to find in fermentations the same organisms as in infusions, since Pasteur nourished his ferments with vegetable infusions; he hesitates, however, on finding that the butyric ferment belongs to the Infusoria. "I was so far," he said, "from expecting such a result, so far, indeed, that for a long -80- |