and working as he had done with anthrax blood, Pasteur saw develop everwhere small non-motile segments of an extreme tenuity, slightly constricted in the middle ( Fig. 22 ), and clearly approaching, much more than the anthrax bacteridium and the other bacilli, those microscopic granules to which Chauveau had attributed the active rôle in the virulent humors of cowpox, smallpox, and sheeppox. Fig. 22. --Microbe of chicken cholera. Young. Old. This organism is so tenuous that the precipitate which it forms at the bottom of the flask is sometimes almost invisible; it appears scarcely to touch the nutri- tive substances placed at its disposal, and one might ask himself the question whether it changes in any respect the culture fluid. "Let us try," said Pasteur to himself; and he tried, and saw with surprise that if this bouillon culture was filtered by passing through a porous wall in order to remove from it all the parasites, and then re-inoculated, no growth took place. The -277- |