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not contain this enzyme. In this way, by association of destructive and non-
destructive methods, both of which are indispensable, it is possible to
demonstrate with certainty that the metabolic sequence is A-B-X.Nevertheless, it can be objected that a product accumulated by one
mutant and utilized by another mutant could very possibly be the product
of a side-reaction.For example, consider the reaction scheme
It is possible to imagine, if the block is between A and B, that an extract
of the wild strain of the micro-organism would covert A' into B whilst an
extract of the blocked mutant would not. Is this a reason for placing A' in
the direct metabolic sequence to X, that is for writing A→ A→ B →X?
No, because the isolation of enzymes enables us to demonstrate that a single
enzyme is capable of converting A into B and that, for the conversion of A'
into B, two enzymes are necessary; in addition to the first, we require an
enzyme catalysing the reaction: A′ →A. This is an illustration of the
importance of studies on purified enzymes, alongside experiments using
non-destructive methods.
REFERENCES
HARRIS H. ( 1953). An Introduction to Human Biochemical Genetics. Univ. Press,
Cambridge.
WAGNER R. P. and MITCHELL H. K. ( 1955). Genetics and Metabolism. Wiley,
New York.

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Questia, a part of Gale, Cengage Learning. www.questia.com

Publication Information: Book Title: Unity and Diversity in Biochemistry: An Introduction to Chemical Biology. Contributors: Marcel Florkin - author, T. Wood - transltr. Publisher: Pergamon Press. Place of Publication: Oxford. Publication Year: 1960. Page Number: 185.
    
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