Photomicrography

Photomicrography

Photomicrography

Photomicrography

Excerpt

Advances in microscopy along many lines have been numerous since the publication of the first edition. New designs of microscopes, cameras, and accessories have made much earlier apparatus obsolete, even though basically the older equipment is in most cases just as efficient when properly used. The trend in photographic equipment has been largely toward universality of application, combined with automatic operation.

Among the advances in theoretical and applied microscopy can be mentioned phase and interference microscopy, electron microscopy, the widespread use of color photography, and the application of polarized light to many types of investigation not previously considered practical or useful. The introduction of Polaroid and its frequent substitution for the more expensive types of calcite prisms has helped to make the use of polarized light more general.

Much of the new apparatus has been so designed that a microscopist no longer need be trained in optical theory when it comes to the operation of his microscope or camera. Integral light sources, properly aligned for critical illumination, are now available in many forms for those who desire to use them. Flexibility in accessory apparatus, at least in the more elaborate equipments, enables one to do all types of microscopical work -- light field, dark field, polarizedlight, phase microscopy, and photography in color in all sizes of film from 16- and 35-mm. up to the capacity of the camera provided -- all this with a complete range of magnifications -- as well as copying, enlarging, reducing, and other ordinary types of photographic work occasionally required in photomicrographic work.

In the optical field there is now a definite trend toward the application of the method suggested by Sir Isaac Newton for eliminating chromatic and spherical aberrations through the use of spherical mirrors as objectives. It can be predicted that future developments along this line will be made and wider application of this type of objectives will result.

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