Academic journal article Forensic Science Communications

Chapter 18: Fiber Examination-UV/VIS Microspectrophotometry

Academic journal article Forensic Science Communications

Chapter 18: Fiber Examination-UV/VIS Microspectrophotometry

Article excerpt

General Discussion

This module is intended to familiarize the trainee with the use of ultraviolet/visible light microspectrophotometry as a qualitative, quantitative and objective method of color analysis. Color analysis can be applicable to natural as well as manufactured fibers. The calculation of complementary chromaticity coordinates is not required for color comparisons. However, the trainee should obtain theoretical knowledge of colorimetry for thoroughness in understanding color analysis.

Objectives

Upon satisfactory completion of this training module, the trainee will have developed and demonstrated theoretical knowledge and/or practical skills to:

1. properly operate and maintain the microspectrophotometer and its accessories;

2. understand the optical properties of the microspectrophotometer;

3. identify those situations in which microspectrophotometry is appropriate;

4. prepare samples and select appropriate mounting media;

5. evaluate the number of fibers required within a control sample to yield representative spectra;

6. interpret and compare spectra; and

7. have an understanding of colorimetry (e.g. color intensity, hue, calculation of chromaticity coordinates). …

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