Academic journal article Bulletin of the World Health Organization

CD Antigens 1993: An Updated Nomenclature for Clusters of Differentiation on Human Cells

Academic journal article Bulletin of the World Health Organization

CD Antigens 1993: An Updated Nomenclature for Clusters of Differentiation on Human Cells

Article excerpt

Introduction

The nomenclature for clusters of differentiation (CD), which is dedicated to the designation of cell surface structures of human cells, was established by the International Workshop on Human Leukocyte Differentiation Antigens and continues to be updated by these Workshops. The CD nomenclature was initially proposed to cluster monoclonal antibodies (mAbs) of different sources that recognize the same human leukocyte differentiation antigen. The nomenclature is based on analysis of the results of blinded studies of the molecular mass of antigens recognized by mAbs submitted for evaluation to the Workshop and of the reactivity of the mAbs with target cells. The nomenclature was aimed to be simple, unambiguous and adaptable.

The CD nomenclature is now widely used to designate the molecular species recognized by a mAb. New mAbs are characterized and therefore new CD antigens are defined after each International Workshop. In addition, the CDw "Workshop" designation is attributed to either (1) a single mAb recognizing the cell surface product of a transfected gene, or (2) a new molecule identified by one or two mAbs generated in the same laboratory. The CD # R symbols are given to indicate that the tissue distribution of CD # may be restricted. In some cases the restriction has been attributed to alternative splicing; in the latter instance, the letter R is followed by a letter corresponding to relevant exons.

Results

The results of the 5th International Workshop on Human Leukocyte Differentiation Antigens were presented on 3-7 November 1993 at a meeting in Boston, MA, USA. The participants represented the efforts of more than 500 laboratories worldwide, which joined together over a two-year period to analyse 1450 antibodies and characterize over 150 molecules. Blind panels for all monoclonal antibodies (mAbs), every CD antigen, every known candidate for CD status, and all mAbs of undefined specificity were analysed by flow cytometry. Other dedicated laboratories undertook serological, molecular, biochemical and histochemical characterization of the mAbs and the structures they defined. The results obtained by all groups showed almost perfect concordance.

Detailed results of these studies will be published separately.(a) In addition, a Leukocyte Differentiation Antigen Database (LDAD) has been developed to (1) provide identifying information on all molecules and mAbs studied in the Workshop, and (2) display/analyse the quantitative expression of each molecule on more than 80 cell types. …

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