Academic journal article Genetics

Corrigenda

Academic journal article Genetics

Corrigenda

Article excerpt

In the article by K. S. Kocherlakota, J.Wu, J.McDermott, and S. M. Abmayr (Genetics 178: 1371-1383) entitled "Analysis of the Cell Adhesion Molecule Sticks-and-Stones Reveals Multiple Redundant Functional Domains, Protein- Interaction Motifs and Phosphorylated Tyrosines That Direct Myoblast Fusion in Drosophila melanogaster," the information in the materials and methods section regarding the snsGal4 construct is incorrect.

The published information states:

The snsGal4 construct wasmade by cloning a 5-kb fragment including 2 kb upstream of the sns genomic region into pPTGAL vector (Sharma et al. 2002). sns-LacZ was generated by introducing the same region into pH-pelican (Barolo et al. 2000).

However, the 5-kb construct did not include 2 kb upstream and, instead, extended further downstream. Potentially important regulatory elements are likely included in intron 1 and would be missed by anyone using the published information to isolate the enhancer region that we used. A corrected statement follows:

The snsGal4 construct wasmade by cloning a 5-kb fragment from the sns genomic region, beginning 550 bp upstream of the Sns start codon and ending at nucleotide position 4482 in intron 1, into pPTGAL (Sharma et al. 2002). The snsLacZ construct was generated by introducing a 3100-bp genomic fragment, which ended at nucleotide position 4482 in intron 1, into pH-pelican (Barolo et al. …

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