Academic journal article Iranian Journal of Public Health

COMPARISON BETWEEN GENE CASSETTES ARRAY OF INTEGRON CLASS I IN MULTIDRUG RESISTANCE ACINETOBACTER BAUMANNII AND KLEBSIELLA PNEUMONIAE IN Iran

Academic journal article Iranian Journal of Public Health

COMPARISON BETWEEN GENE CASSETTES ARRAY OF INTEGRON CLASS I IN MULTIDRUG RESISTANCE ACINETOBACTER BAUMANNII AND KLEBSIELLA PNEUMONIAE IN Iran

Article excerpt

Background: Integrons are considered to play a major role in multidrug resistance dissemination among gram-negative bacteria. These are genetic elements capable of integrating and mobilizing individual gene cassettes via a site-specific recombination mechanism.Antibiotic resistance in Acinetobac- ter baumannii and Klebsiella penumoniae is a major problem in the hospital and outbreaks caused by this organism have been reported frequently. The present study aimed at determining the prevalence of different classes of integrons and the char- acterization of integron class 1 gene cassettes in Iranian A. baumannii and K. penumoniae isolates.

Methods: A total of 63 multidrug resistance A. baumannii and 100 K. penumoniae isolates were collected from clinical specimens in the Vali-Asr hospital in the central province of Iran. The antimicrobial susceptibility for 15 antibiotics was determined by disk diffusion. The presence of different in- tegron classes was investigated by PCR and the size of gene cassettes in class 1 integrons was then determined by PCR as well. Moreover, integron cassette arrays of isolates were de- lineated by RFLP and sequencing amplicons with different lengths.

Results: 90% of K. pneumoniae and 98.4% A. baumannii iso- lates carried class 1 integrons, whereas class 2 integrons were found in 2% and 15.9% of K.pneumoniae and A. …

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