Academic journal article Iranian Journal of Public Health

Some Morphologic and Molecular Properties of Native Sinorhizobium Meliloti Bacteria from Khorasan-Razavi

Academic journal article Iranian Journal of Public Health

Some Morphologic and Molecular Properties of Native Sinorhizobium Meliloti Bacteria from Khorasan-Razavi

Article excerpt

Background: The aim of this study was to investigate about some morphologic and molecular properties of native Sino- Rhizobium meliloti bacteria from Khorasan-Razavi.

Methods: For this purpose, bacteria were isolated from the roots of alfalfa plants that were collected from some areas of Khorasan- Razavi and examined for some biochemical prop- erties. Morphologic properties were evaluated using stere- omicroscope and electromicroscope. The plasmid profile of isolates was studied by Chen method. Molecular identifica- tion carried out by polymerase chain reaction (PCR) using specific primers of 16Sr DNA (rd1: 5'-AGAGTTT- GATCCTGGCTCAG-3', fd1: 5'-AAGGAGGTGAC- CAGCC-3'). The PCR product of one sample was sequenced on both strands by using fD1and rD1 primers with an ABI 3730 XL automated DNA sequencer.

Results: All nine isolated bacteria were found to Gram-neg- ative asporogenous rods and coccobasili. Their colonies were convex, mucoid and white to cream color and the diameter of colonies was sometimes different. …

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