Academic journal article Iranian Journal of Public Health

A Comparative Study of Antimicrobial Activity of Nisin Loaded in Alginate and Alginate-Resistant Starch Microparticles

Academic journal article Iranian Journal of Public Health

A Comparative Study of Antimicrobial Activity of Nisin Loaded in Alginate and Alginate-Resistant Starch Microparticles

Article excerpt

Background: This study was designed to compare antimi- crobial activity of alginate and alginate-resistant starch micro- particles against Listeria monocytogenes.

Methods: Nisin loaded microparticles were prepared by a w/o-emulsion external cross-linking procedure with three- weight ratio of nisin to alginate (4: 1, 2: 1 and 1: 1). For com- parison of the antimicrobial activity of nisin encapsulated in alginate and alginate-resistant starch microparticles, the re- lease of nisin from microparticles was studied in distilled water as release medium and activity of released nisin was determined using the food pathogen Listeria monocytogenes ATCC 19117in agar diffusion test. Three wells of 6 mm di- ameter were punched into the agar on each plate (that seeded by 100µl of an overnight broth culture containing 107-108 CFU/ml of the test organisms) and lyophilised microcapsules were loaded into wells. Plates were incubated at 37 °C for 24 h. The diameter of inhibition zone was then measured. The amount of released nisin from microparticles was determined using bacteriocin standard curve.

Results: The nisin release from all formulations occurs in two stages: first, a rapid release phase, which corresponds to nisin being physically entrapped in the suface of microparti- cles, and second, the nisin being gradually released, until the nisin content was constant (66-76% in Alg microparticles and 61-70% in Alg-RS microparticles). …

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