Academic journal article Iranian Journal of Public Health

Comparison of Cage and Baba Virulence Genes Expression in Spiral and Coccoid Forms of Helicobacter Pylori

Academic journal article Iranian Journal of Public Health

Comparison of Cage and Baba Virulence Genes Expression in Spiral and Coccoid Forms of Helicobacter Pylori

Article excerpt

Background: Under stressful environment, a spiral form of H. pylori is transformed into a coccoid form. The coccoid formation is very important problem in recurrence of disease and antibiotic treatment failure in gastritis pateints. Our ob- jective of this study was to determine the morphological transformation-process from spiral to a coccoid form under Amoxicillin stress and also to evaluate and comparison of the virulence genes (cagE and babA) expression in spiral and coccoid forms of H. pylori.

Methods: The standard ATCC 26695 H. pylori strain and 9 clinical isolates were used in this study. The existence of in- terest genes were detected by conventional PCR method. Minimal inhibitory concentration (MIC) of amoxicillin was determined using by E-test method. All of ten isolates were cultured under MIC, ½, ¼ and 1/8 MIC concentrations of amoxicillin. The induction of coccoid forms was examined periodically by gram staining and light microscopy. Then, the viability of induced coccoids was evaluated by flow cytometry method. The quantitative mRNA expression levels of cagE and babA genes were detected and compared in spiral and coccoid forms of H. pylori by real-time PCR method. …

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