Academic journal article Iranian Journal of Public Health

A COMPARATIVE STUDY ON DIAGNOSTIC ASSAYS, PCR, MICROSCOPIC AGGLUTINATION TEST (MAT) AND CULTURE FOR DETECTION OF RODENT LEPTOSPIROSIS IN MAZANDARAN PROVINCE, Iran

Academic journal article Iranian Journal of Public Health

A COMPARATIVE STUDY ON DIAGNOSTIC ASSAYS, PCR, MICROSCOPIC AGGLUTINATION TEST (MAT) AND CULTURE FOR DETECTION OF RODENT LEPTOSPIROSIS IN MAZANDARAN PROVINCE, Iran

Article excerpt

Background: Leptospirosis is caused by infection with Lep- tospira bacteria. The infection transfers from animals (com- monly rats, cattle, pigs and dogs) to humans. Transmission occurs by direct or indirect contact with serum, urine or oth- er biological fluids of infected animals. Thus, animals (espe- cially rodents) can act as a source of infection for humans or other animals. It has a worldwide epidemic, but most in tem- perate and tropical regions especially in areas with rainfall and neutral and slightly alkaline soil such as northern Iran.

Methods: Rodents were trapped in 10 different areas of Ma- zandaran Province during the summer 2013, and 150 rodents were captured alive. Urine and kidney samples were collected from each rodent and then, samples were used for culture in accordance for isolation of live Leptospira. The presence of Leptospira DNA was evaluated in urine and kidney samples from all mice using nested PCR method. In sera samples, antibody titers and possible types of infecting serovarieties were tested by Microscopic Agglutination Test (MAT) using a panel of 20 strains of live Leptospira as antigens. The statisti- cal analysis of the obtained data was done using SPSS version 19. A 'Pvalue'<0.05 was considered to be statistically signifi- cant.

Results: Live Leptospira were isolated from the kidney and urine samples of 3 mice (2%). In nested PCR method, DNA of Leptospira was found just in the one urine sample of rodent (0.7%) but it was detected from kidney samples of 16 rodents (10. …

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