Academic journal article Iranian Journal of Public Health

Intranasal Immunization with Recombinant Fimh Fused with Flagellin Enhances Immunity against Urinary Tract Infection

Academic journal article Iranian Journal of Public Health

Intranasal Immunization with Recombinant Fimh Fused with Flagellin Enhances Immunity against Urinary Tract Infection

Article excerpt

Background: In this study, we evaluated the humoral im- munity of recombinant fusion FimH.FliC of UPEC as a vac- cine candidate against UTI by intranasal route.

Methods: The constructed FimH.FliC fusion protein was purified by Ni-NTA column and then LPS of the protein was removed by Triton x114. Mice were intranasally immunized three times with 25 µg of proteins (FimH, FliC or FimH.FliC proteins). Total IgG, IgG isotypes (IgG1, IgG2a), and IgA responses of immunized mice were measured by ELISA.

Results: All mice groups induced significant immune re- sponse against FimH and FliC as compared to control mice. Mice immunized with the fused FimH.FliC protein induced significantly higher humoral (Total IgG, IgG1, IgG2a and IgA) immune responses than with FimH alone or FimH ad- mixed with FliC. Our results showed that based on the IgG1/ IgG2a ratios, FliC directed the anti-FimH responses preferentially towards Th2. …

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