Academic journal article Iranian Journal of Public Health

SEQUENCE VARIATION OF PERTUSSIS TOXIN S1 SUBUNIT GENE IN CLINICAL BORDETELLA PERTUSSIS STRAINS OF IranIAN ISOLATES

Academic journal article Iranian Journal of Public Health

SEQUENCE VARIATION OF PERTUSSIS TOXIN S1 SUBUNIT GENE IN CLINICAL BORDETELLA PERTUSSIS STRAINS OF IranIAN ISOLATES

Article excerpt

Background: According to the importance of pertussis vac- cine and the lack of fundamental studies about the polymor- phism of virulence genes in Iran, the aim of the present search is investigation of s1 pertussis toxin gene polymor- phism on circulating strains compared to vaccine strains.

Methods: 50 nasopharyngeal Dacron swabs from patients with a clinical diagnosis of pertussis sent to pertussis refer- ence laboratory at the Pasteur Institute of Iran in 2008-2012. The cultivation, biochemical tests, the antiserum, and PCR were used to confirm B. pertussis. Sequencing of PCR prod- ucts was performed to determine ptxS1 alleles. Two strains of BP 134 and BP 18323 as vaccine strains were studied.

Results: All 50 clinical isolates of B. pertussis were studied. All strains had the dominant allele ptxS1A. There was difference between the alleles of clinical strains and vaccine strains. …

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