Academic journal article Iranian Journal of Public Health

The White Line Formation, a Remarkable Defensive Strategy of Pseudomonas on the Battlefield

Academic journal article Iranian Journal of Public Health

The White Line Formation, a Remarkable Defensive Strategy of Pseudomonas on the Battlefield

Article excerpt

Background: The purpose of our main researches has been to obtain genetic and functional characterization of WLIP lipopeptide and the interesting phenomenon of defensive white-line formation in Pseudomonads. This unique strategy of Pseudomonas could be useful in controlling clinical pathogens.

Methods: A mutagenesis approach combined with draft genomic sequencing (next generation sequencing) as well as in silico analyses was employed. In parallel, WLIP was ex- tracted, purified and subjected to MS and NMR analyses for structural elucidation and confirmation.

Results: Although the structure of WLIP has been known for more than 2 decades, genetic analysis of its biosynthesis was not yet reported. We have characterized the WLIP-syn- thesizing NRPS system of P. putida RW10S2 and P. fluo- rescens LMG 5329. Both systems have several features in common, including three NRPSs genes located in two re- mote genomic regions, an export system and a LuxR-type regulator. …

Search by... Author
Show... All Results Primary Sources Peer-reviewed

Oops!

An unknown error has occurred. Please click the button below to reload the page. If the problem persists, please try again in a little while.