Academic journal article Iranian Journal of Public Health

A Recombinant Subunit Vaccine Based on the L7/l12 and Tomp31 Proteins Induces Protection against Brucella Infection in Balb/c Mice

Academic journal article Iranian Journal of Public Health

A Recombinant Subunit Vaccine Based on the L7/l12 and Tomp31 Proteins Induces Protection against Brucella Infection in Balb/c Mice

Article excerpt

Background: Brucellosis is the most common bacterial zo- onotic disease worldwide and no vaccine is available for the prevention of human brucellosis. In humans, brucellosis is mostly caused by Brucella melitensis and Brucella abortus. The Omp31 and L7/L12 are immunodominant and protective antigens conserved in human Brucella pathogens. The aim of this study was to evaluate the immunogenicity & protective efficacy of a recombinant protein vaccine encoding Brucella truncated Omp31 and L7/L12 proteins.

Methods: Bioinformatic tools were used to design the trun- cated Omp31 and L7/L12- TOmp31 fusion protein. The humoral/cellular immune response and protection levels against challenge with wild B. melitensis and B. abortus were evaluated in vaccine immunized mice and control groups.

Results: Vaccination of BALB/c mice with the recombinant fusion protein (rL7/L12-TOmp31) provided the significant protection level against both B. …

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