Academic journal article Iranian Journal of Public Health

Bioremediation of Nickel through Metarhizium Sp. Utmc 5002

Academic journal article Iranian Journal of Public Health

Bioremediation of Nickel through Metarhizium Sp. Utmc 5002

Article excerpt

Background: In this study, we isolated nickel resistance fun- gi and evaluated their potential for bioremediation appli- cation in acidic pH condition.

Methods: Soil samples were collected from heavy metal con- taminated parts of Iran. Fungal colonies were isolated and purified on potato dextrose agar (PDA), pH 5.7, amended with 400 mg/l of nickel. The plates were incubated at 28 °C for 2 weeks. Purified isolates were tested for tolerance against 1000 mg/l of Ni in PDB, pH 2.5 with incubation at 28 °C for 7 days. Selected strains were screened for ability to grow in PDB containing 1000 mg/l of Ni, pH 1, 1.5 and 2. For mo- lecular characterization of the final selected isolate, genomic DNA extraction and subsequently, PCR amplification using nu-ssu-1536 and nu-ssu-817 primers was performed. PCR product was sequenced by Macrogen. The sequence was compared with those of other validated species using BLAST program.

Results: Using Ni supplemented medium and cultivation conditions described before, a total of 120 fungal colonies were obtained from15 soil samples. Most of the isolates showed heavy growth on PDA plates. The entire isolates were preserved in University of Tehran Microorganisms Col- lection (UTMC). During the primary screening of the whole 120 purified isolates, 11 strains enabled to tolerate 1000 mg/l of Ni in acidic pH (2. …

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