Academic journal article Iranian Journal of Public Health

Over-Production of Epidermal Growth Factor (Egf) in E. Coli

Academic journal article Iranian Journal of Public Health

Over-Production of Epidermal Growth Factor (Egf) in E. Coli

Article excerpt

Background: Epidermal Growth Factor (EGF) is a mono- mer polypeptide, which is produce from different tissue in human. It has many applications in medicine and Pharma- ceutical Science. The main source of EGF is Parotid gland. Production of EGF from animal's source has many disad- vantages and it is not suitable for human applications. The best way for production of this protein is recombinant DNA technology, batch fermentation and High cell density culture for E. coli. In this study, we report the heterologus over-ex- pression of EGF in E. coli BL21 (DE3).

Methods: Escherichia coli is the most widely used host for producing recombinant proteins. Using BL21 derived strain can reduce proteolyses of heterologus protein. In this study we increased several times recombinant human EGF pro- duction in E. coli BL21 (DE3) [pET28a-egf] by optimization media and temperature in induction duration. For this reason the transformed BL21 (DE3) with pET28a including syn- thetic gene kinetic cell growth and rhEGF production inves- tigated at three different medium like LB, TB and 32Y with different temperatures such as 24, 28, 32 and 37°C. The re- sults of experiments were analyzed by SDS-PAGE, and cell growth and recombinant protein production kinetics.

Results: The optimal expression of EGF in E. coli can be easily achieved when the growth conditions are properly con- trolled. …

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