Academic journal article Iranian Journal of Public Health

UNIQUE BIODIVERSITY OF RADIATION RESISTANT BACTERIA ISOLATED FROM AN IranIAN RADIOACTIVE SITE AND ANALYSIS OF THEIR PIGMENTS

Academic journal article Iranian Journal of Public Health

UNIQUE BIODIVERSITY OF RADIATION RESISTANT BACTERIA ISOLATED FROM AN IranIAN RADIOACTIVE SITE AND ANALYSIS OF THEIR PIGMENTS

Article excerpt

Background: Radiation resistance has been detected dis- persedly among bacteria and some microorganisms growing in natural radioactive environments. Herein, we provide data of isolation, molecular identification and pigments characteri- zation of radiation resistant bacteria recovered from an Ira- nian radioactive site.

Methods: The soil samples were collected from uranium radioactive site and the physico-chemical properties of the soil samples were determined. Soil serial dilutions were cul- tured in Trypton-Glocose-Yeast extract agar and all distinct colonies irradiated by gamma and ultraviolet radiation at var- ious doses using a 60Co source. The surviving bacteria were identified by morphology, biochemistry and 16S rRNA gene sequencing. In addition, bacterial pigments were analyzed by spectroscopic properties, HPLC and LC-MS systems and their antioxidant activity were assessed by DPPH activity.

Results: Among 30 pure colonies, five UV and gamma radi- ation resistant bacteria were isolated from a radioactive site. Phylogenetic analysis based on 16S rRNA gene sequencing reveal that the isolated strains were belonged to Micrococcus lylae, Kocuria rhizophila, Rhodococcus erythropolis and Dermacoccus nishinomiyaensis. …

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