Academic journal article Iranian Journal of Public Health

Study of Expression of the Gene Alpha-6 in Multidrug-Resistant Acinetobacter Baumannii against Thyme Essence with Real Time Pcr

Academic journal article Iranian Journal of Public Health

Study of Expression of the Gene Alpha-6 in Multidrug-Resistant Acinetobacter Baumannii against Thyme Essence with Real Time Pcr

Article excerpt

Background: The present study was aimed to investigate the inhibitory effects of Thyme essence on the expression of antibiotic resistance genes aphA-6 and Housekeeping DNA gyrase -A against Multidrug-resistant strains of Acinetobacter baumannii with Real time PCR technique.

Methods: Five Multidrug-resistant strains of Acinetobacter baumannii among seventy-five drug-resistant strains of Aci- netobacter baumannii selected from hospitals in Tehran. AphA- 6 and Housekeeping DNA Gyrase-A genes with PCR meth- od were approved for studies. Acinetobacter baumannii ATCC #19606 was used in this study as a model reference strain. MIC values were determined by broth micro dilution assay recommended by the NCCLS. Thyme essence to the MIC was added to 1 ml Muller Hinton broth and after mixing, 1 ml of bacterial suspension (5 × 10 5 CFU / ml) was added to the medium, incubated at 37 ° C for 24 hours. After incubat- ing bacteria, bacterial mRNA was extracted and transformed into cDNA and level of expression of aphA-6 and House- keeping DNA gyrase-A genes in comparison to non-exposed Thyme essence was examined by Real time PCR.

Results: The major components of Thyme essence were thymol (28.8%) and carvacrol (23.46%). Thyme essence with MIC (0.45 l / ml) and an inhibitory effect on multidrug- resistant A.bummanii was found. The average zone of inhibi- tion by Thyme essence on multidrug-resistant A. …

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