Academic journal article Iranian Journal of Public Health

Study of Class-D Oxacillinase Types in Imipenem-Resistant Acinetobacter Baumannii Clinical Isolates by Rapd-Pcr

Academic journal article Iranian Journal of Public Health

Study of Class-D Oxacillinase Types in Imipenem-Resistant Acinetobacter Baumannii Clinical Isolates by Rapd-Pcr

Article excerpt

Background: The objective of this study was to investigate the genetic fingerprints of class D oxacillinase positive imipenem-resistant Acinetobacter baumannii clinical isolates.

Methods: Sixty clinical isolates of A. baumannii collected from Imam Hossein and Shahid Motahari Hospitals between October 2011 to April 2012 were employed. Presence of class D oxacillinases was shown by PCR and genotyping was performed using random amplified polymorphic DNA (RAPD-PCR). Correlation between class D gene carriage and RAPD-PCR patterns was studied among the isolates.

Results: Majority of the imipenem-resistant isolates carried class D oxacillinase genes of which, 80% carried OXA-23, 60% had OXA-51, 61.66% harbored OXA-58 and 20% of the isolates carried OXA-24. It should be noted that in most isolates there was more than one gene. RAPD-PCR revealed three clusters (A-C) on a similarity level of 70% among which 53. …

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