Academic journal article Iranian Journal of Public Health

Molecular Identification of Esbl Genes Bla -Shv, Bla-Veb, and Bla-Per, in Acinetobacter Baumannii Isolated from Patients Admitted in a University Hospital in Isfahan

Academic journal article Iranian Journal of Public Health

Molecular Identification of Esbl Genes Bla -Shv, Bla-Veb, and Bla-Per, in Acinetobacter Baumannii Isolated from Patients Admitted in a University Hospital in Isfahan

Article excerpt

Background: Acinetobacter baumannii has become one of the most important pathogens responsible for healthcare-associated infections and particularly affects pa- tients admitted to intensive care units (ICUs). Prevalence of class extended-spectrum -lactamases (ESBLs) has been inves- tigated in Acinetobacter baumannii. The aims of this study were to determine the prevalence of ESBL genes bla SHV, bla VEB, and bla PER in clinical isolates of Acinetobacter bau- mannii in a university hospital in Isfahan.

Methods: Within 8 mounths, 121 clinical isolates of A. bau- mannii were collected from Alzahra Hospital in Isfahan. To confirm the species of baumannii a PCR test for identification of blaoxa-51 genes was conducted. Antimicrobial susceptibil- ity was done by testing resistance to cefotaxime, ceftriaxone, ampicillin-sulbactam, cefepime, meropenem,, tobramycin, amikacin, tetracycline, ciprofloxacin, trimethoprim sulfa- methoxazole, Aztreonam by using the Kirby Bauer disk dif- fusion method. Finally all of isolates were evaluated by PCR for detection of bla-SHV, bla-PER and bla-VEB genes.

Results: Among 121 isolates in this study 44% were female and 56% were male. Samples were cultured from the Trachea (35%), urine (17%), and blood (10%). …

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