Academic journal article Iranian Journal of Public Health

Preparation and in Vitro Evaluation of Antitumor Activity of Tgfal3-Seb as a Ligand-Targeted Superantigen

Academic journal article Iranian Journal of Public Health

Preparation and in Vitro Evaluation of Antitumor Activity of Tgfal3-Seb as a Ligand-Targeted Superantigen

Article excerpt

Background: In this study, we explored the antitumor po- tency of tumor-targeted super antigens (TTS) strategy; we designed and produced a fusio n protein as a new antitumor candidate by fusing the third loop (L3) of transforming growth factor α (TGFαL3) genetically to staphylococcal en- terotoxin type B and evaluated in vitro antitumor activities against murine breast cancer cell line.

Methods: We designed and prepared TGFαL3-SEB chimer- ic protein and evaluated superantigenic activity, binding property to cancer cell over expressing epidermal growth factor receptor (EGFR) and in vitro antitumor activities.

Results: Cloning of tgfαl3-seb was confirmed by colony- PCR, enzymatic digestion and sequencing. The recombinant TGFαL3-SEB fusion protein with molecular weights of 31 kDa was expressed and confirmed by anti-his western-blot analysis. TGFαL3-SEB fusion protein attached to A431 cell line with proper affinity and induced dose-dependent cyto- toxicity against cancer cells expressing EGFR in vitro. …

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