Academic journal article Iranian Journal of Public Health

Optimization of Benzene Biodegradation Using Streptomyces Isolated from Tabriz Refinery

Academic journal article Iranian Journal of Public Health

Optimization of Benzene Biodegradation Using Streptomyces Isolated from Tabriz Refinery

Article excerpt

Background: Streptomyces bacteria isolated from soil of Ta- briz Refinery was used for biodegradation of benzene. Then kinetic modeling of biodegradation and optimization of envi- ronmental conditions and pH were performed.

Methods: After isolation and screening, effective species for degradation of benzene were detected. Then bacteria were cultured in Muller Hinton Agar. Mineral medium was used for experiments. In addition to benzene, bacteria were added to one of the samples and by comparison of mineral medium containing only benzene (control environment); the changes resulting from the influence of bacteria were investigated. The ability of bacteria to decompose benzene was deter- mined by maximum wavelength using spectrophotometer. Further experiments were carried out using gas chromatog- raphy. After extracting experimental data, the reaction kinetic model for biodegradation was proposed and optimization of the concentration, temperature and pH was performed.

Results: Bacteria can play an important role in bioremedia- tion and cleaning hydrocarbon contaminated soils due to their ability for biodegradation of benzene. …

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