Academic journal article Iranian Journal of Public Health

Purification and Determination of Optimum Ph and Temperature of Intracellular L-Asparaginase from Bacillus Sp.pg-02

Academic journal article Iranian Journal of Public Health

Purification and Determination of Optimum Ph and Temperature of Intracellular L-Asparaginase from Bacillus Sp.pg-02

Article excerpt

Background: Bacterial L-asparaginases are amidohydrolases that catalyze the conversion of L-asparagine to L-aspartate and ammonia. The enzymes isolated from E. coli and Erwinia carotovora are now being used in the treatment of acute lym- phoblastic leukemia. However, prolonged administration of L-asparaginase causes an anaphylactic shock or neutralization of the drug effect. Therefore there is a continuing need to screen newer organisms in order to obtain strains capable of producing new and high yield of L-asparaginase. In the pre- sent study a novel strain, Bacillus sp.PG-02 was explored for the production of intra-cellular L-asparaginase.

Methods: Bacillus sp.PG-02 was grown in a modified M9 medium and incubated in a rotary shaking incubator. After 24 h of inoculation, the cells were removed by centrifugation. Sonication of the cells was carried out to release the intracel- lular L-asparaginase and DEAE-Cellulose Ion exchange chromatography with discontinuous gradient of NaCl was used for purification of enzyme. The optimum pH and tem- perature for L-asparaginase activity were determined. …

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