Academic journal article Iranian Journal of Public Health

Production of Pectinase by Aspergillus Niger Using Solid-State Substrates

Academic journal article Iranian Journal of Public Health

Production of Pectinase by Aspergillus Niger Using Solid-State Substrates

Article excerpt

Background: The purpose of this research was to optimize the production of pectinase by solid substrate fermentation using lignocellulosic wastes (wheat straw, rice straw) and pec- tic substance such as citrus pulp (lemon, orange).

Methods: Aspergillus niger PTCC 5010 was obtained from Iranian Research Organization for Science and Technology (IROST). Taguchi design was employed for screening the most significant factors affecting the pectinase production by strain under study. The effect of addition of various solid- state substrates such as wheat straw, rice straw, orange pulp and lemon pulp, pulp ratio, carbon / nitrogen (C/N) ratio, and pH was studied for optimal pectinase production. Pecti- nase assay was done spectrophotometrically using galacturon- ic acid (GaiA) as the substrate.

Results: In present study mixed of wheat and rice straws was utilized in combination with orange and lemon pulp with various percentages to product the enzyme with Aspergillus niger. The results showed that the highest pectinase activity was obtained using lemon pulp and orange pulp with maxi- mum activity of 7819.15 U/mg. …

Search by... Author
Show... All Results Primary Sources Peer-reviewed

Oops!

An unknown error has occurred. Please click the button below to reload the page. If the problem persists, please try again in a little while.