Academic journal article Iranian Journal of Public Health

Evaluation of Pyrite Removal from Mouteh Refractory Gold Ore Using Acidithio Bacillus Ferrooxidans Ptcc1647 in Semi Industrial Scale

Academic journal article Iranian Journal of Public Health

Evaluation of Pyrite Removal from Mouteh Refractory Gold Ore Using Acidithio Bacillus Ferrooxidans Ptcc1647 in Semi Industrial Scale

Article excerpt

Background: The aim of this work is to evaluate the pyrite remove from mouteh gold ore by A. ferrooxidans PTCC1647 in column.

Methods: In the first step, Acidithio Bacillus Ferrooxidans PTCC1647 purchasad from (Iranian Research Organization for Science and Technology(IROST) was cultivated in the routinely 9k medium. In the second step, the cells suspen- sions (2x108 cells/ml) were used as inoculum at the 10 %v/v for column bioleaching. then column(75cm/7.5cm) was charged with 4Kg of sulfide gold ore from Mouteh gold mine isfahan province (Iran) with particle size 45 mesh(3.36 mm) containing FeS2(pyrite)78 %. 9K medium with initial pH 1.80 Containing pure cultures of A. Ferrooxidans PTCC1647 was pumped from the feed container to the top of the column. Air was pumped from Top of the column into holes embed- ded within the column. Experiments were carried out at 30 °C for 60 days with pumping speed 1.8 l/h. Moreover, sterile column was prepared as the uninoculated control system. Redox potential, pH, ferrous iron concentaration(by spectro- photometer using 1, 10 orthophenanthroline ferrous complex as an indicator) and Sulfate concentration(indirectly deter- mined by atomic absorption spectroscopy analysis of Ba after precipitation of BaSO4) were measured daily. The chemical composition and particle size distribution of ore was deter- mined after bioleaching experiments. …

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