Academic journal article Iranian Journal of Public Health

Isolation and Characterization of Phenol- Degrading Bacteria from the Arak Petrochemical Complex

Academic journal article Iranian Journal of Public Health

Isolation and Characterization of Phenol- Degrading Bacteria from the Arak Petrochemical Complex

Article excerpt

Background: Phenol is an environmental pollutant present in industrial wastewaters such as refineries, coal processing and petrochemicals industries. Bioremediation has become one of the most rapidly developing fields of environmental restoration, utilizing microorganisms to reduce the concen- tration and toxicity of various chemical pollutants. This ap- proach represents a good alternative compared with physico- chemical strategies, which have high costs and produce other toxic products.

Methods: Phenol degrading bacteria were isolated from acti- vated sludge of Arak Petrochemical Complex by serial dilu- tions of enriched consortium. In order to assess the utiliza- tion rates of phenol using the isolated strains, microbial inoc- ulums were passed to the mineral salt medium with phenol (1000mg/l) as the sole carbon and energy source. The effi- ciency of isolated strains was assessed by monitoring the bac- terial growth (OD600nm) and phenol biodegradation (spec- trometrically using 4-aminoantipyrene). …

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