Academic journal article Iranian Journal of Public Health

Evaluation of Crude Oil Biodegradation and Laccase Activity in Newly Isolated Phaeosphaeria Sp Utmc 5003

Academic journal article Iranian Journal of Public Health

Evaluation of Crude Oil Biodegradation and Laccase Activity in Newly Isolated Phaeosphaeria Sp Utmc 5003

Article excerpt

Background: The present study was carried out to investi- gate Iranian indigenous fungal strains to remove crude oil pollutants.

Methods: First, for the fungal strain isolation, the contami- nated soil samples were collected from different habitats of Iran. The soil samples were enriched in Minimal Salt Medium (MSM) supplemented with 1% crude oil, and chlorampheni- col. Purification of isolates was carried out on PDA media. Afterward, each of pure fungal isolates were evaluated for growth rate and degradation ability after 15 days under 1% crude oil in MSM, by measuring the dry weight, total petrole- um hydrocarbons (TPH) assay at 420 nm and the residual hydrocarbon content by FTIR. In the following, laccase ac- tivity of this isolate in the presence of 1% crude oil was measured. Finally, the best isolate was identified based on 18s rRNA gene sequence analysis and its morphology of colony and microscopic examination with the reference to identifica- tion keys.

Results: 30 different fungal strains were isolated. TPH test and gravimetric growth of each isolate in MSM supplemented with 1% crude oil as the sole carbon source showed that the isolate G-05 with 65% degradation over 15 days, was the best isolate in removing the petroleum hydrocarbons. …

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