Academic journal article Iranian Journal of Public Health

ISOLATION OF PHYTASE-PRODUCING BACTERIA FROM RHIZOSPHERE OF IranIAN WHEAT

Academic journal article Iranian Journal of Public Health

ISOLATION OF PHYTASE-PRODUCING BACTERIA FROM RHIZOSPHERE OF IranIAN WHEAT

Article excerpt

Background: In this study, phytase-producing bacteria from7 cultivar of rhizosphere wheat (Chamran, Tajan, Morvarid, Pishtaz, Oroum, Sivand, Zarea) were solated and identified based on morphological and biochemical character- istics.

Methods: Enzyme-producing bacteria were determined by production of clear zones around the colonies on the medi- um containing sodium phytate, after 24-48 h of incubation at 30°C. Because some bacteria in phytase specific medium are able to create false positive results,we eliminated them by phytase plate assay (cobalt chloride, ammonium molybdate / ammonium vanadate). The best phytase producing isolates were recognized by its surrounding clear halo.

Results: Out of 47 colonies on media plates, 19 colonies showed positive for phytase production and created zone of clearing around microbial colonies, but 11 false positive col- onies were detected. …

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