Academic journal article Iranian Journal of Public Health

Nutrient and Physical Variables Optimization of Phb Production by Alcaligenes Eutrophus

Academic journal article Iranian Journal of Public Health

Nutrient and Physical Variables Optimization of Phb Production by Alcaligenes Eutrophus

Article excerpt

Background: Polyhydroxyalkanoates (PHAs) are completely biodegradable thermoplastics, which are accumulated as an energy reserve material by a large number of bacteria in the presence of excess carbon source and limitation of nitrogen or phosphorous sources. In the present study, efforts have been made to optimize the effect of various nutritional and physical parameters for enhanced PHB production by Alcali- genes eutrophus PTCC 1615 using Plackett-Burman and Re- sponse Surface Methodology statistical methods.

Methods: In the present study, efforts have been made to optimize the effect of various nutritional and physical param- eters for enhanced PHB production by Alcaligenes eutrophus PTCC 1615 using Plackett-Burman and Response Surface Methodology statistical methods. At first, based on one- factor-at-a-time experiments, fructose and ammonium chlo- ride were found to be the most suitable sources of carbon and nitrogen for PHB production.

Results: Then through the Plackett-Burman and central composite design fructose, agitation speed, KH2PO4, and initial pH were recognized as the most significant factors affecting PHB accumulation. …

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