Academic journal article Iranian Journal of Public Health

Study of Organophosphorus Insecticide Chlorpyrifos Biodegradation by Halo-Tolerant Bacteria Isolated from Contaminated Agricultural Lands

Academic journal article Iranian Journal of Public Health

Study of Organophosphorus Insecticide Chlorpyrifos Biodegradation by Halo-Tolerant Bacteria Isolated from Contaminated Agricultural Lands

Article excerpt

Background: Because many agricultural lands in Iran are saline, aim of this project is to investigate the biodegradation of cloropyrifos as an Organophosphorus (OP) compound model by halo-tolerant bacteria isolated from agricultural land.

Methods: Soil and water samples were collected from saline agricultural areas with history use of OP. For isolation of bacteria capable of utilizing CP as sole carbon source, en- richment technique with the modified-M9 medium supple- mented with 50 ppm CP and 5% NaCl was used. The time of each round of enrichment was 14 days. After three rounds of enrichment, modified R2A agar medium (with 5 % NaCl) was used for isolation and purification of single colonies. To select the isolates with higher resistance to CP toxicity and CP-degraders that are more efficient the growth of selected strains in modified-M9 media containing 100 to 500 ppm CP as sole source of carbon was assayed after 7 days with UV- visible spectrophotometer at 600nm. Plate-count method was used to compare the growth rate of the isolates in presence of 500 ppm CP.

Results: After three rounds of enrichment, 28 morphologi- cally different bacterial colonies were isolated and purified. Evaluation of the growth of the isolates in the presence of 100 ppm CP after 7 days, led to selection of 5 isolates for further studies. …

Search by... Author
Show... All Results Primary Sources Peer-reviewed

Oops!

An unknown error has occurred. Please click the button below to reload the page. If the problem persists, please try again in a little while.