Academic journal article Iranian Journal of Public Health

Cytocidal Actions of Parasporin, an Anti-Tumor Crystal Toxin from Bacillus Thuringiensis

Academic journal article Iranian Journal of Public Health

Cytocidal Actions of Parasporin, an Anti-Tumor Crystal Toxin from Bacillus Thuringiensis

Article excerpt

Background: Parasporin, a new crystal protein derived from non-insecticidal and non-hemolytic Bacillus thuringiensis, rec- ognizes and kills human colon cancer cells as well as some classes of human cultured cells. Here we report that a novel cytotoxic protein was isolated from the crystal produced by B. thuringiensis subsp. thuringiensis S9 strain.

Methods: Fifty soil samples were collected from different Iranian provinces, and characterization was performed based on protein crystal morphology by phase-contrast microscope and variations of Cry protein toxin using SDS-PAGE. After parasporin process with proteinase K, the active form was produced and protein activity on the cell line was evaluated.

Results: Parasporal inclusion proteins showed different cyto- toxicity against human colon cancer cell line (HCT-116), but not against normal lymphocyte. Isolated parasporin demon- strated no hemolytic activity against human erythrocyte. It appears that these proteins have the ability to differentiate between normal lymphocytes and leukemia cells and have specific receptors on specific cancer cell lines. …

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