Academic journal article Iranian Journal of Public Health

Evaluation of Microbial Life in Salt Lake Razazeh, Karbala - Iraq

Academic journal article Iranian Journal of Public Health

Evaluation of Microbial Life in Salt Lake Razazeh, Karbala - Iraq

Article excerpt

Background: The aim of this research was to evaluate halo- philic bacterial diversity in Lake Razazeh as regards this is the first report on halophilic bacteria isolation in Iraq.

Methods: Samples of saline soils, mud and water were col- lected from 15 locations of Lake Razazeh with distance of 4 km from each other. Halophiles were isolated by saline nutri- ent broth enrichment. The growth was diluted 10 times and plated on complete medium agar with a salt gradient of 2.5 percent. Cultures were incubated at pH 7-7.5 and 37°C dur- ing 7 days given that all medium were controlled every day. Gram staining was carried out as a routine initial procedure in the identification of unknown bacterial species.

Results: Two hundred and eighteen isolates were selected from isolation plates, which were named K1-K218. There were 161 gram-positive rod and cocci and 57 gram-negative rods. More than fifty strains were isolated using the morpho- logical differences based on visible examination of the growth characteristics, and were used for further analysis. Also, 15 protozoa, 3 algae, 5 fungi and yeast were observed in the culture medium. …

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