Academic journal article Iranian Journal of Public Health

Screening for Uricase Enzyme from Halotolerant Bacteria

Academic journal article Iranian Journal of Public Health

Screening for Uricase Enzyme from Halotolerant Bacteria

Article excerpt

Background: Uricase (urate oxidase, EC 1.7.3.3) is the en- zyme that catalyzes specifically the oxidation of uric acid to allantoin and plays an important role in nitrogen metabolism. Uric acid and its salts are relatively insoluble in water, easily precipitated, and the abnormal accumulation of uric acid is a causative factor of gout in humans. The aim of this study was screeinning for new resoureces for uricase; to be most effec- tive as well as commercially valuable.

Methods: All strains investigated in this study isolated from different saline and hypersaline lakes and wetlands from Iran. A total of 65 halotolerant strains were tested in this project. Strains were cultured on a production medium containing 0.3 % uric acid and total salt 3 %. The agar plates were incubated at 30?C and monitored 48 h. A clear zone indicates the pres- ence of uricase enzyme. Strains with clear zone were selected for quantitative assay with UV visible-spectrophotometer at 293nm. …

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