Academic journal article Iranian Journal of Public Health

The Prevalence of Chlamydia Trachomatis in Patients with Benign and Malignant Ovarian Cancer by Nested Pcr Method

Academic journal article Iranian Journal of Public Health

The Prevalence of Chlamydia Trachomatis in Patients with Benign and Malignant Ovarian Cancer by Nested Pcr Method

Article excerpt

Background: The aim of this study was to investigate the prevalence of C.trachomatis in patients with ovarian cancer that referred to Imam Hossein Hospital in Tehran, Iran.

Methods: In this descriptive study that was conducted from January 2013 to April 2014, 120 pathological samples were studied which obtained from patients with ovarian cancer who referred to Imam Hossein Hospital. The samples were transferred to the laboratory of Shahid Beheshti University of Medical Sciences. DNA was extracted with QIAamp blood mini kit (Qiagen,USA) according to manufacturer's instruc- tions. In order to detect the presence of C.trachomatis, spe- cific primers for the MOMP (Major Outer Membrane Pro- tein) genes of C.trachomatis were designed and evaluated by Nested PCR method.

Results: Out of 120 samples of ovarian cancer,60 (50%) samples were malignant cancer and 60 (50%) were benign cancer as control group. Results of Nested PCR showed that 14(23. …

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