Academic journal article Iranian Journal of Public Health

Detection of Chlamydia Trachomatis in Fertile and Infertile Women in Sanandaj by Pcr

Academic journal article Iranian Journal of Public Health

Detection of Chlamydia Trachomatis in Fertile and Infertile Women in Sanandaj by Pcr

Article excerpt

Background: The aim of this study was to determine the prevalence of C. trachomatis in infertile and fertile women in Sanandaj (Kurdistan, Iran).

Methods: 104 infertile women referring to Clinic-affiliated Besat hospital in Sanandaj (Kurdistan, Iran) from February to May 2013 were selected as the case group and 104 fertile women with at least one child who referred to non-infertility clinics at the same time were included as the control group. Dacron Swabs were used for sampling from the endocervical canal of women. Cervical swabs were transported to labora- tory in 5 mL of Phosphate Buffered Saline (PBS) medium and were frozen at -20°C until examination. DNA was ex- tracted from samples using DNA extraction kit and subjected to Polymerase chain reaction (PCR) using C. trachomatis spe- cific primers. Statistical analysis was performed using the statistical package for the social sciences (SPSS) software version 13.0 for Microsoft windows.

Results: The age ranging for both groups was 14-40 (average age 31.63 years for fertile group and 29.16 years old for infer- tile patients). In fertile group 6 cases (5.76 %) were positive for C. trachomatis while infertile group, C. trachomatis was de- tected from 5 patients (4.80 %).

Conclusion: Prevalence of C. trachomatis infection in the two studied groups was almost the same and no significant differ- ence between fertile and infertile groups was observed. …

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