Academic journal article Iranian Journal of Public Health

Characterization of Chlamydia Trachomatis Omp1 Genotypes Isolated from Patients with Follicular Conjunctivitis Referred to Farabi Hospital by Rflp

Academic journal article Iranian Journal of Public Health

Characterization of Chlamydia Trachomatis Omp1 Genotypes Isolated from Patients with Follicular Conjunctivitis Referred to Farabi Hospital by Rflp

Article excerpt

Background: In this study, we used Omp1 Genotyping for analysis of samples from patients with follicular conjunctivitis referred to Farabi Hospital.

Methods: Conjunctival scraps (n=90) were obtained from patients who were referred to the diagnostic laboratory of Farabi hospital during 2012. Smears were prepared by rolling half the swab on to the center of a glass slide. Smears were fixed and used for direct immunofluorescence (DIF) method using a genus specific fluorescein isothyocyanate labelled Chlamydia monoclonal antibody. In Giemsa stain, diagnosis was based on the prescience of inclusions that were baso- philic and stained pinkish -blue. PCR amplification after ex- traction was performed using CT1 and CT5 primers designed from Omp1 gene. Omp1 Genotyping was performed by RFLP method using AluI.

Results: Among 90 cases examined for Chlamydia trachomatis in eyes, 28 patients (31.1%) were positive by DIF and 13 (14.4%) by Giemsa staining and 35(38.8%) showed positive result in PCR. …

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