Academic journal article Iranian Journal of Public Health

Isolation and Genotyping of Clostridium Perfringens from Broiler Meat in Mashhad City

Academic journal article Iranian Journal of Public Health

Isolation and Genotyping of Clostridium Perfringens from Broiler Meat in Mashhad City

Article excerpt

Background: Clostridium perfringens is an important cause of bacterial food poisoning worldwide. The disease is caused by C. perfringens enterotoxin (CPE) encoded by cpe gene. The aim of this research was to identify the different types of C. perfringens and the presence of cpe gene in isolated bacteria from broiler meats presented in retail meat shops of Mash- had city in northeastern of Iran.

Methods: In this study, 200 samples of fresh chicken car- casses were purchased, randomly from different retail meat shops. In laboratory after rinsing the carcasses and culturing in enrichment and selective media, morphological test was performed from suspected colonies. After DNA extraction from the suspected colonies, for confirming the isolates as C. perfringens, a PCR assay using specific primers for 16s rRNA gene, and for toxin typing a multiplex PCR assay with specif- ic primers, were performed. …

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