Academic journal article Iranian Journal of Public Health

Adhesion Site of Botulinal Toxin: Is It a Proper Vaccine Candidate?

Academic journal article Iranian Journal of Public Health

Adhesion Site of Botulinal Toxin: Is It a Proper Vaccine Candidate?

Article excerpt

Background: The botulinum neurotoxins are the causative agents of botulism and represent a family of seven structural- ly similar but antigenically distinct serotypes (A to G). The toxins exert their action by blocking the release of the neuro- transmitter acetylcholine at the neuromuscular junction. The toxin consists of a 100 kDa heavy chain and 50 kDa light chain held together by a single disulfide bond. The carboxy- terminal (Hc) fragment of botulinum toxin B (BHc) in E. coli strain GM2163 was expressed and evaluated in this study.

Methods: All strains of E. coli used in this study were pri- marily cultured on LB broth. The appropriate media were supplemented with ampicillin. DNA fragments which had been generated using the PCR and the synthetic gene was digested cloned into Hind III and Nco I digested plasmid PTG19-T and transformed into E. coli strain GM2163. The nucleotide sequence of the cloned fragment was determined to ensure authenticity. …

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