Academic journal article Iranian Journal of Public Health

Incidence of Clostridium Perfringens and Clostridium Difficile in Patients with Gastrointestinal Disorders

Academic journal article Iranian Journal of Public Health

Incidence of Clostridium Perfringens and Clostridium Difficile in Patients with Gastrointestinal Disorders

Article excerpt

Background: We undertook this study to investigate the presence of C. perfringenes in two groups of diarrheal patients (IBD and non- IBD patients).

Methods: A total of 97 stool samples were obtained from patients with gastroenteritis, including 55(5.85%) from non- IBD patients and 39 (41.5%) from IBD patients. The stool samples were examined for the detection of C. perfringenes and C. difficile after treatment with buffer salin phosphate. The treated samples were cultured on Neomycin Egg Yolk Agar plates supplemented with 5% horse blood and CCFA for isolation of C. perfringens and C. difficile, respectively and incu- bated at 37 °C under anaerobic conditions for 2 days. PCR was done on the extracted DNAs of C. difficile and C. perfringenes colonies for toxin encoding genes tcdA, tcdB and cpe.

Results: Out of the 97 stool samples tested, C. …

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