Academic journal article Iranian Journal of Public Health

MOLECULAR STUDY OF BRUCELLOSIS IN ENDEMIC REGIONS OF Iran BASED ON NOVEL URS-PCR METHOD

Academic journal article Iranian Journal of Public Health

MOLECULAR STUDY OF BRUCELLOSIS IN ENDEMIC REGIONS OF Iran BASED ON NOVEL URS-PCR METHOD

Article excerpt

Background: Brucellosis is an infectious disease in human and animals. Bacteriologic-culture-based methods used for Brucella diagnosis are hard and time consuming. The aim of this study was to introduce a novel molecular method for detection of two prevalent species of Brucella in Iran, Brucella abortus and Brucella melitensis

Methods: All complete sequences of chromosome 1 with 2.1 Mbp length were compared with all available Brucella se- quences. A unique loci was found in chromosome one which was capable to differentiate Brucella abortus from Brucella melitansis. A set of primers was designed in flanking of unique loci. A total number of 136 (88 human and 48 bovine) well-characterised Brucella strains have been evaluated and classified by PCR method.

Results: Results of biochemical tests and bacteriophage typ- ing as golden standard indicated that all Brucella strains isolat- ed from human and bovine were B. melitensis biovar 1 and B. abortus biovar 3 respectively. The PCR results were the same as bacterilogical method in detection of the Brucella species. …

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