Academic journal article Iranian Journal of Public Health

Isolation, Identification of Xanthomonas Strain from Leaves of Lemon Trees and Studying the Production of Xanthan Gum

Academic journal article Iranian Journal of Public Health

Isolation, Identification of Xanthomonas Strain from Leaves of Lemon Trees and Studying the Production of Xanthan Gum

Article excerpt

Background: Xanthan gum is an important microbial poly- saccharide, which is synthesized by bacteria of the genus Xanthomonas and considered as pest for a lot of plants. This research has been done with the purpose of isolating and identifying Xanthomonas strain from leaves of lemon trees and the production of Xanthan gum.

Methods: Sampling from marked leaves of lemon trees that were infected by bacterial canker was performed and isolating the disease agent on culture environment of YDC was carried out at 28 °C temperature after 24-48 hours. Then, the ob- tained colony was assessed for morphological and biochemi- cal characteristics viewpoints. An environment for producing Xanthan gum was used to produce the gum and then final identification of separated bacteria was studied by 16S rRNA method.

Results: In this research, a new strain under the name of Xanthomonas campestris strain saba. …

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