Academic journal article Iranian Journal of Public Health

Direct Identification of Streptococcus Agalactiae at Vaginal Colonization in Pregnant Women by Pcr

Academic journal article Iranian Journal of Public Health

Direct Identification of Streptococcus Agalactiae at Vaginal Colonization in Pregnant Women by Pcr

Article excerpt

Background: Streptococcus agalactiae is a commensal organism but it may cause infection in susceptible hosts including new- borns, pregnant or postpartum women. Applying rapid, accu- rate, and sensitive method for detecting GBS and receiving intrapartum antibiotic prophylaxis (IAP) at delivery have been demonstrated to increase treatment possibility of carrier pregnant women and decrease the rates of GBS vertical transmission to infants.The aim of this study was to evaluate PCR assay targeting 16S rRNA primers compared with con- ventional culture method for direct detection of GBS in vag- inal specimens of pregnant women at 35-37 weeks of gesta- tion in Hamadan.

Methods: 203 vaginal specimens of pregnant women at 35- 37 weeks of pregnancy from June 2013- February 2014 were evaluated for detection of GBS using culture method and PCR assay.

Results: The prevalence of GBS in 203 collected samples was 7.39% using culture method and 19.70% using PCR as- say. 25 specimens were determined to be positive by PCR and negative by culture; 2 specimens were positive by culture and negative by PCR. Generally, a total of 42 specimens (20. …

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