Academic journal article Iranian Journal of Public Health

Comparison of Four Diagnostic Methods for Detection of Methicillin-Resistant Staphylococcus Aureus

Academic journal article Iranian Journal of Public Health

Comparison of Four Diagnostic Methods for Detection of Methicillin-Resistant Staphylococcus Aureus

Article excerpt

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a well-known pathogen with a worldwide distribu- tion. Due to the increasing rate of MRSA infections, imple- menting of reliable, accurate and rapid testing for diagnosis of MRSA is necessary. The aim of this study was to compare four diagnostic methods for detection of MRSA isolates.

Methods: From December 2012 to April 2014, we collected 120 S. aureus isolates from three hospitals affiliated with Teh- ran University of Medical Sciences. MRSA isolates were de- tected by four different methods including cefoxitin disc dif- fusion test, oxacillin disc diffusion test, minimum inhibitory concentration (MIC) of oxacillin as determined by MIC test strip, and mecA detection by PCR.

Results: Out of 120 S. aureus isolates, cefoxitin disc diffusion test, oxacillin disc diffusion test and MIC test strip identified 60 (50%), 48 (40%), 55 (45.83%) isolates as MRSA, respec- tively. …

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