Academic journal article Iranian Journal of Public Health

Development and Clinical Validation of Loop-Mediated Isothermal Amplification Method for Rapid Detection of Bordetella Pertussis Based on Insertion Sequence Is481

Academic journal article Iranian Journal of Public Health

Development and Clinical Validation of Loop-Mediated Isothermal Amplification Method for Rapid Detection of Bordetella Pertussis Based on Insertion Sequence Is481

Article excerpt

Background: In spite of the existence of conventional cul- ture method and developing PCR-based assays, yet the accu- rate detection of Bordetella pertussis is a problem. In this study, we developed a Loop-Mediated Isothermal Amplification (LAMP) assay based on IS481 and it has evaluated the effi- ciency, rapidity, sensitivity, and specificity of IS481 LAMP in comparison with real-time PCR with the same target for the detection of B. pertussis clinical specimens.

Methods: One hundred and sixty-five nasopharyngeal swabs were used to be assessed by LAMP and real-time PCR. Am- plified LAMP products were detected by adding SYBR® Safe DNA Gel Stain. Illumination under UV light and the green color for positive samples were visible by unarmed eyes.

Results: The sensitivity and specificity of the LAMP assay were reasonable in comparison with real-time PCR. …

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