Academic journal article Iranian Journal of Public Health

PLASMID-MEDIATED QUINOLONE RESISTANCE DETERMINANTS QNR AND AAC(6')-IB-CR IN FLUOROQUINOLONE-RESISTANT ENTEROBACTERIACEAE IN HAMADAN, Iran

Academic journal article Iranian Journal of Public Health

PLASMID-MEDIATED QUINOLONE RESISTANCE DETERMINANTS QNR AND AAC(6')-IB-CR IN FLUOROQUINOLONE-RESISTANT ENTEROBACTERIACEAE IN HAMADAN, Iran

Article excerpt

Background: The quinolone group is an important class of broad-spectrum antimicrobial agents. Plasmid mediated quinolone resistance (PMQR) determinants have emerged as a significant concern in recent years. This study reports the screening of resistant-isolates to Fluoroquinolone antimicro- bial agents for PMQR determinants and detection of Qnr and aac(6')-Ib-cr genes.

Methods: Total of 100 fluoroquinolone-resistant Enterobac- teriaceae were isolated from 3 hospitals in Hamadan from October 2012 to June 2013. The isolates were identified by biochemical tests and confirmed by PCR. Antimicrobial sus- ceptibility to 14 antimicrobial agents including levofloxacin and ciprofloxacin was determined by Clinical Laboratory Standards Institute (CLSI).Disk diffusion methods and ciprofloxacin MIC were obtained by broth microdilution. Then the isolates were screened for the presence of qnrA, qnrB, qnrS and aac(6')-Ib-cr genes using PCR assay.

Results: Among the screened isolates, 64 strains (64%) of Escherichia coli, 23 strains (23%) of Klebsiella pneumoniae, 13 strains (13%) of Proteus mirabilis were collected as quino- lone-resistant isolates. …

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