Academic journal article Iranian Journal of Public Health

FREQUENCY OF MUTATIONS ASSOCIATED WITH RIFAMPICIN RESISTANCE IN MYCOBACTERIUM TUBERCULOSIS STRAINS ISOLATED FROM PATIENTS FROM WEST OF Iran

Academic journal article Iranian Journal of Public Health

FREQUENCY OF MUTATIONS ASSOCIATED WITH RIFAMPICIN RESISTANCE IN MYCOBACTERIUM TUBERCULOSIS STRAINS ISOLATED FROM PATIENTS FROM WEST OF Iran

Article excerpt

Background: Tuberculosis (TB) is a devastating infectious disease causing high mortality and morbidity worldwide. The most serious threat related to tuberculosis control is the re- cent emergence of drug-resistant tuberculosis strains. The aim of the present study was to identify various types of mu- tations in rpo B region from rifampicin resistant strains iso- lated from sputum of tuberculosis patients.

Methods: Drug susceptibility testing of 125 Mycobacterium tuberculosis isolates was determined using the CDC standard conventional proportional method. Target DNA of M. tuber- culosis was amplified by PCR, hybridized and scanned. We used the LCD array to detect mutations within the 90 bp rpoB region. Each array is a transparent, pre-structured pol- ymer supports using a non-fluorescent detection principle based on the precipitation of a clearly visible dark substrate.

Results: Of the 125 M. tuberculosis isolates, 35 (28%) were found to be rifampicin resistant and using the LCD array revealed point mutations at 9 different codons as follows S512T(AGC[arrow right]ACC) (20%) D516V (GAC[arrow right]GTC)(20%) H 526D (CAC[arrow right]GAC) (6%)H526R(CAC[arrow right]CGC)(20%) H526Y (CAC[arrow right]TAC) (23%) S531W (TCG[arrow right]TGG)(8%). …

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