Academic journal article Iranian Journal of Public Health

Resistance to Tetracycline and Vancomycin of Staphylococcus Aureus Isolates from Sanandajian Patients

Academic journal article Iranian Journal of Public Health

Resistance to Tetracycline and Vancomycin of Staphylococcus Aureus Isolates from Sanandajian Patients

Article excerpt

Background: The increase of Staphylococcus aureus resistance to antibacterial drugs is one of the major health concerns, therefore studying antibiotic resistance of Staphylococcus aureus is very important and it has a main role in preventing the emergence of resistant strains. This study was done to deter- mine the prevalence and genetic basis of tetracycline and vancomycin resistance in Staphylococcus aureus in Sanandaj city.

Methods: One hundred and fifty clinical isolates of S. aureus were collected from Sanandaj Hospital. Susceptibility to anti- biotics (tetracycline and vancomycin) was determined by disk agar diffusion method with minimal inhibitory concentration (MIC) evaluated on Muller-Hinton agar as described by the Clinical and Laboratory Standards Institute (CLSI). The tet- racycline and vancomycin strains were screened by polymer- ase chain reaction (PCR) for the presence of five common vancomycin and tetracycline resistance determinants, respec- tively, van A, tet K, tet M, tet L and tet O.

Results: Using the DAD method, 12% of the Staphylococcus aureus isolates were resistant to vancomycin and 61/33% to tetracycline. …

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